Measurement of Cell Adhesion and Migration Using Phosphor-Screen Autoradiography

Measurement of Cell Adhesion and Migration Using Phosphor-Screen Autoradiography

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This study demonstrates the use of phosphor-screen autoradiography as a means of measuring cell adhesion and cell expansion on polymer surfaces.The method has particular merit in cases where a specific substrate may be opaque or biochemically incompatible with colorimetric assay methodologies.With the phosphor-screen autoradiography method, there was a linear relationship between korpskaft cell number and quantitated radioactivity.

The technique has also been validated by comparison with a colorimetric assay of adhesion conducted for attachment to conventional culture substrata.The data supported the view that the use of phosphor-screen autoradiography was a valid method for detecting cell attachment, and it gave equivalent results to the colorimetric assay.Furthermore, a comparison between phosphor-screen autoradiography and a dye-staining method showed that this technique can be truvisionhealthftp.com used as a means of quantifying cellular expansion over surfaces.